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Two novel Drosophila genes, CG32702 and CG11592, are required for nephrocyte function. (A and B) Secreted ANF-RFP (red) is accumulated in pericardial nephrocytes labeled with Hand-GFP (green). (C–F) Pericardial nephrocyte-specific RNAi knockdown of (C and D) CG32702 or (E and F) CG11592 completely blocked the accumulation of ANF-RFP (red), but the survival of pericardial nephrocytes was not affected based on Hand-GFP (green) expression in the nephrocytes.
