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. 2013 Jan 30;8(1):e55407. doi: 10.1371/journal.pone.0055407

Figure 7. Effect of antioxidants and buthionine sulfoximine (BSO) on the entry of DV2 into HepG2 cells.

Figure 7

Confluent monolayers of HepG2 cells were infected with DV2 at 1 of multiplicity of infection (MOI). Medium containing Glutathione (GSH) or BSO was incubated with HepG2 cells during the virus adsorption period. Then, the cells were washed three times and freeze-thawed to release intracellular virus. Virus titers were detected by using the plaque assay and were expressed as a percentage of the DV2-infected group that did not receive treatment. DV2: DV2 infected HepG2 cells (MOI = 1), DV2+GSH: DV2 infected HepG2 cells with GSH treatment of 10 mM or 20 mM, DV2+BSO: DV2 infected HepG2 cells with BSO treatment of 0.2 mM or 1 mM. Values shown are means ± S.D. from five independent experiments. (**p<0.01 v.s. DV2 group).