TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| M182 | Δlac | 5 |
| JRG4830 | M182 Δlac Δcrp | 5 |
| JRG4864 | M182 Δlac Δhns; Cmr | This work |
| JRG2631 | M182 Δlac Δfnr Δcrp; Tetr | Jeff Cole |
| JRG4865 | M182 Δlac Δfnr Δhns; Cmr Tetr | This work |
| JRG4732 | M182 Δlac Δcrp Δhns; Cmr | This work |
| JRG4866 | M182 Δlac Δfnr Δcrp Δhns; Cmr Tetr | This work |
| JRG4747 | M182 Δlac Δfnr; Cmr | This work |
| JRG3212 | M182 Δlac Δfnr Δcrp; Cmr | This work |
| JRG4385 | JM109 (pGS1482); Apr | 32 |
| Plasmids | ||
| pRS415 | Medium-copy-number vector (15 to 20 copies per cell; pBR322 origin of replication) for construction of hlyE::lacZ reporter plasmid pGS1629; Apr | 29 |
| pGS1629 | pRS415 containing PhlyE (−299 to +82); Apr | This work |
| pRW50 | Low-copy-number (2 to 5 copies per cell; pSC101 origin of replication), lac reporter vector; Tetr | 15 |
| pGS1065 | pRW50 containing PhlyE (−97 to +61); Tetr | 10 |
| pGS1064 | pUC118 containing PhlyE (−97 to +61, start codon is +1); Apr | 10 |
| pGS1482 | pGEX-KG (13) containing the slyA coding region; Apr | 32 |
| pGS1657 | pBluescript containing S. enterica slyA coding region and promoter; Apr | This work |