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RNA dot blot. Total RNA from cells of KIM6+ (Hms+), KIM6 (Δpgm), and KIM6-2051+ (hmsT2051::mini-kan) cultured at 26 or 37°C were transferred to nylon membranes and hybridized against probes for hmsH (A), hmsS (B), or hmsT (C). RNase indicates hybridization against 1 μg of RNA treated with RNase A.
