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Mutations in hmsR, hmsS, and hmsT do not affect levels of HmsH protein after growth at 26 or 37°C. Equal concentrations of whole-cell lysates were separated by SDS-PAGE; immunoblots were reacted with the antiserum against HmsH. HmsS is not produced in KIM6-2012 due to polarity of the mini-kan insertion into hmsR, which is upstream of hmsS.
