FIG. 7.

Western blot analysis of expression of HmsH, HmsF, HmsR, HmsS, and HmsT proteins from Y. pestis KIM6+ (Hms⁺) and KIM6 (Δpgm; i.e., Hms⁻) or KIM6-2051+ (hmsT2051::mini-kan) cells grown at 26 or 37°C. Equal concentrations of whole-cell lysates were separated by SDS-PAGE; immunoblots were reacted with the antiserum against individual Hms proteins. Relevant proteins are labeled. HmsT is encoded outside the pgm locus and therefore still expressed in KIM6 cells; consequently, KIM6-2051+ cell extracts were used as a negative control.