TABLE 2.

β-Galactosidase activities of Y. pestis containing either the hmsHFRS::lacZ or hmsT::lacZ reporter^a

Strain	Mean β-galactosidase activity: (Miller units) of cells grown atb:
	26°C in deferrated PMH2			37°C in deferrated PMH2			26°C in HIB			37°C in HIB
	NAc	10 μM FeCl3	10 μM Hemin	NA	10 μM FeCl3	10 μM Hemin	Exponential	Early stationary	Late stationary	Exponential	Early stationary	Late stationary
KIM6-2097 (hmsHFRS::lacZ)	11,192 ± 1,653	8,422 ± 1,733	7,614 ± 1,618	15,012 ± 2,861	13,180 ± 2,530	11,772 ± 3,054	3,411 ± 426	5,438 ± 769	8,959 ± 2,023	4,584 ± 589	6,709 ± 444	8,526 ± 1,127
KIM6-2096+ (hmsT::lacZ)	7,071 ± 1,281	6,686 ± 2,840	5,873 ± 1,985	9,198 ± 2,437	7,933 ± 993	7,410 ± 1,156	2,325 ± 478	2,724 ± 787	4,256 ± 763	6,056 ± 1,584	7,499 ± 3,708	12,844 ± 1,068

^aCells were harvested during exponential, early-stationary, and late-stationary phases of growth (HIB cultures) or only during exponential-phase growth (PMH2 cultures).

^bEnzyme activities are expressed in Miller units (33). The values shown (mean±standard deviations) are the average of two or more individual reactions from three or more independent cultures.

^cNA, no additions