TABLE 5.
Expression profiles of genes of lysine biosynthesis during batch cultivation of lysine producing C. glutamicum ATCC 13287a
| Enzyme | EC no. | Expression (relative level ± SD) at indicated time
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| 5.63 h | 6.22 h | 6.48 h | 6.75 h | 6.85 h | 7.02 h | 7.25 h | 7.73 h | ||
| Aspartate aminotransferase | 2.6.1.1 | 1.66 | 1.42 ± 0.05 | 0.96 | 0.62 ± 0.02 | 0.35 | ND | 0.73 | 0.93 ± 0.05 |
| Aspartate kinase | 2.7.2.4 | 0.80 ± 0.04 | 0.82 ± 0.02 | 1.06 ± 0.07 | 1.13 ± 0.02 | 1.08 ± 0.05 | 1.22 ± 0.01 | 1.15 ± 0.00 | 1.18 ± 0.05 |
| Aspartate semialdehyde DH | 1.2.1.11 | 0.99 ± 0.09 | 0.87 ± 0.09 | 1.03 ± 0.05 | 0.96 ± 0.09 | 1.02 ± 0.02 | 1.02 ± 0.01 | 1.14 ± 0.09 | 1.30 ± 0.06 |
| Dihydrodipicolinate synthase | 4.2.1.52 | 0.95 ± 0.02 | 1.21 ± 0.00 | 1.20 ± 0.09 | 1.05 ± 0.05 | 0.93 ± 0.12 | 1.00 ± 0.07 | 1.00 ± 0.05 | 1.34 ± 0.19 |
| Dihydrodipicolinate reductase | 1.3.1.26 | 0.98 ± 0.11 | 0.95 ± 0.13 | 1.20 ± 0.08 | 1.16 ± 0.05 | 1.27 ± 0.10 | 1.34 ± 0.29 | 0.95 ± 0.06 | 0.96 ± 0.07 |
| Succinyl transferase | 2.3.1.117 | 0.70 ± 0.06 | 0.57 ± 0.06 | 0.78 ± 0.13 | 0.65 ± 0.05 | 0.85 ± 0.20 | 0.70 ± 0.03 | 0.85 ± 0.13 | 0.84 ± 0.04 |
| Succinyl diaminopimelate DS | 3.5.1.18 | 1.06 ± 0.33 | 0.92 ± 0.02 | 1.27 ± 0.29 | 0.98 ± 0.03 | 0.81 ± 0.07 | 1.09 ± 0.06 | 1.04 ± 0.24 | 1.35 ± 0.03 |
| Diaminopimelate epimerase | 5.1.1.7 | 0.63 ± 0.01 | 0.62 ± 0.03 | 0.83 ± 0.08 | 1.02 ± 0.08 | 0.85 ± 0.09 | 0.82 ± 0.02 | 0.76 ± 0.03 | 0.91 ± 0.01 |
| meso-Diaminopimelate DH | 1.4.1.16 | 0.84 ± 0.09 | 1.20 ± 0.01 | 1.07 ± 0.07 | 0.94 ± 0.17 | 1.02 ± 0.09 | 0.72 ± 0.05 | 0.83 ± 0.07 | 0.91 ± 0.01 |
| Diaminopimelate decarboxylase | 4.1.1.20 | 1.74 ± 0.08 | 1.38 ± 0.09 | 1.09 ± 0.01 | 0.83 ± 0.03 | 0.89 ± 0.07 | 0.84 ± 0.01 | 0.76 ± 0.01 | 0.61 ± 0.03 |
| Lysine exporter regulator | 0.34 ± 0.01 | 0.88 ± 0.12 | 1.38 ± 0.22 | 1.04 ± 0.02 | 1.32 ± 0.00 | 1.28 ± 0.12 | 1.45 ± 0.26 | 1.33 ± 0.08 | |
| Lysine exporter | 0.32 ± 0.03 | 0.61 ± 0.03 | 1.14 ± 0.02 | 1.44 ± 0.11 | 1.48 ± 0.09 | 1.70 ± 0.28 | 1.14 ± 0.05 | 1.22 ± 0.03 | |
a
Gene expression is shown for different time points of the cultivation relating to growth phase (5.63 h), phase shift (6.22 and 6.48 h), and lysine production (6.75, 6.85, 7.02, 7.25, and 7.73 h) and is expressed as relative expression. The deviation between two replicate measurements is shown, except for measurements where only one value was available. ND, not detected.