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. 2013 Jan 30;8(1):e55115. doi: 10.1371/journal.pone.0055115

Figure 3. Pathogen resistance phenotypes of coi1-40 related to JA.

Figure 3

coi1-40 and its controls were tested for: (A) Methyl jasmonate (MeJA) induced resistance. 17-day-old-plants were treated with either 100 µM MeJA (with 0.1% DMSO and 0.02% Silwet L-77) or a mock solution. One day later, Pto was inoculated and its growth measured as in Figure 1A. (B) Systemic Acquired Resistance. Three leaves of 28-day-old plants were hand infiltrated with either Pto(avrRpm1) or a mock solution. Two days later, Pto was inoculated and its growth in systemic leaves measured as described (see Methods). (C) Coronatine as a virulence factor. Bacteria with coronatine (Pto, COR+) or without coronatine (Pto(cfa−), COR−) were inoculated and their growth measured as in Figure 1A. (D) Negative crosstalk with SA. Plants of the indicated genotypes were treated four times with either 350 µM benzothiadiazole (BTH) or a mock solution, and their weight was recorded when 21 days old. The ratio between BTH treated and mock treated is shown as a percentage, with npr1 included as control. jin1 is included in all the panels as a control of no response to JA. Asterisks indicate statistically significant differences from the mock treatment (P<0.05 one asterisks, P<0.01 two) using the Student's t-test (two-tails).