Figure 4. Response of coi1-40 to JA in vitro.

coi1-40 and its controls were tested for: (A) Phenotype in plates. The indicated genotypes were grown in plates with Johnson's Media [67] supplemented with 50 µM MeJA. The pictures were taken 20 days after germination with the same settings and in the same experiment. In plates without MeJA the plants were the same size (data not shown). (B) Length of primary root. The plants were grown as described in (A), with and without 50 µM MeJA. At 10 days old, the lengths of the roots were measured in both conditions, and their ratio (MeJA treated divided by mock treated) expressed as a percentage. (C) Lateral roots. The plants were grown as described in (A), with and without 50 µM MeJA. At 14 days old, the number of lateral roots longer than 0.2 mm was counted in both conditions with the help of a magnifying glass. Note that in some genotypes like Col-0, the root does not grow in MeJA and therefore it is not possible to count lateral roots (marked as “0” in the figure). Since coi1-1 is not fertile, the number of lateral roots without MeJA was not counted (marked as not determined -n.d.- in the figure). (D) Phenotype of F1s between coi1-40 and Col-0 and between coi1-40 and coi1-1. The plants were grown as described in A, with 50 µM MeJA. The pictures were taken when the plants were 20 days old.