Table 1. Negative ion charge deconvoluted electrospray ionization mass spectral data of the core oligosaccharides released by mild acid hydrolysis from LPS of A. hydrohila AH-3ΔwahD, A. hydrohila AH-3ΔwahD (PBAD33-Gm-APJL_0999), A. hydrohila AH-3ΔwahC, and A. hydrohila AH-3ΔwahC (pBAD33-Gm-APJL_1001).
Strain | Molecular mass (Da) | Proposed composition | |
observed | calculateda | ||
AH-3ΔwahD | 1311.44 | 1311.43 | anh-Kdo, 4 Hep, Hex, HexN |
AH-3ΔwahD +APJL_ 0999 | 1937.59 | 1937.58 | anh-Kdo, 6 Hep, 2 Hex, HexN, P |
1857.62 | 1857.61 | anh-Kdo, 6 Hep, 2 Hex, HexN | |
1695.57 | 1695.56 | anh-Kdo, 6 Hep, Hex, HexN | |
1503.50 | 1503.50 | anh-Kdo, 5 Hep, Hex, HexN | |
AH-3ΔwahC | 1665.58 | 1665.55 | anh-Kdo, 5 Hep, 2 Hex, HexN |
1503.53 | 1503.50 | anh-Kdo 5 Hep, Hex, HexN | |
1311.46 | 1311.43 | anh-Kdo, 4 Hep, Hex, HexN | |
AH-3ΔwahC + APJL_1001 | 1937.60 | 1937.58 | anh-Kdo, 6 Hep, 2 Hex, HexN, P |
1857.63 | 1857.61 | anh-Kdo, 6 Hep, 2 Hex, HexN | |
1695.57 | 1695.56 | anh-Kdo, 6 Hep, Hex, HexN | |
1503.50 | 1503.50 | anh-Kdo, 5 Hep, Hex, HexN |
Average mass units (in daltons) used for calculation of molecular masses based on the proposed composition, as follows: anh-Kdo, 202.048; Hep, 192.063; Hex, 162.053; HexN, 161.069; and P, 79.966.