Table 4. Primers used to amplify and subclone individual genes in pBAD33-Gm.
| Primersa,b | Amplified Fragment |
| opsX (APL_0186 and APJL_0187) | |
| AXf: 5’-TGCTCTAGATAATCAGACCGAACCCACGC-3’ | |
| AXr: 5′-CCCAAGCTTCGGCCGGGGTGAGTAATA-3’ | AXf-AXr (1243 bp) |
| wahF (APL_0421 and APJL_0446) | |
| AF2f: 5’-TGCTCTAGATACCGAGCAAGCGGTCAAAT-3’ | |
| AF2r: 5’-CCCAAGCTTGACTTGCCATTTTAACAGGCTTT-3’ | AF2f-AF2r (1185 bp) |
| wahD (APL_0979 and APJL_0999) | |
| ADf: 5’-TGCTCTAGATTAGTCCCGGCTCGGAAATC-3’ | |
| ADr: 5’-CTGCAGTGCCCCAGCTCTTGTTCAAT-3’ | ADf-ADr (1219 bp) |
| wahC (APL_0981 and APJL_1001) | |
| AC2f: 5’-TGCTCTAGATGTGCGCATGGATTTTACGG-3’ | |
| AC2r: 5’-CTGCAGTGTCCTGGATTCAAGCGGTC-3’ | AC2f-AC2r (1155 bp) |
| waaC (APL_1402) | |
| AC11f: C: 5’-TGCTCTAGACGACCGCTTGTTCGTATTCAT-3’ | |
| AC11r: D: 5’-CCCAAGCTTGCAAGCCTCTAATGCAGGAAC-3’ | AC11f-AC11r (1198 bp) |
| waaC (APJL_1427) | |
| AC12f: 5’-TGCTCTAGATGCAGGTTTAGACCGCTTG-3’ | |
| AC12r: 5’-CCCAAGCTTTCACACGCGGTTTGCG-3’ | AC12f-AC12r (1167 bp) |
| waaF (APL_1403 and APJL_1428) | |
| AF1f: 5’-TGCTCTAGATGACCGCTTGTTATTTTAGGGA-3’ | |
| AF1r: 5’-CTGCAGTGACCGCTTGCAATTAGCCT-3’ | AF1f-AF1r (1082 bp) |
a
Bold, underlined, and italic letters XbaI, HindIII, and PstI restriction sites, respectively.
b
The PCR amplified products were digested with the indicated restriction enzymes and ligated to XbaI-HindIII or XbaI-PstI digested pBAD33-Gm.