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L6 myotubes were transfected with either scrambled (scr) or nexilin specific siRNA (si-nex) oligos. Serum depleted cells were stimulated with 100 nM insulin A) or 10 nM B) for the indicated times. IRS1 was immunoprecipitated from cell lysates and complexes probed with either 4G10, nexilin or p85α PI3K abs as indicated.
