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. 2004 Mar;186(6):1658–1666. doi: 10.1128/JB.186.6.1658-1666.2004

FIG. 4.

FIG. 4.

DotB is a hexamer. (A) Superose 12 elution profile for purified wild-type DotB. Milli-absorbance units (mAU) are shown as a function of elution volume. DotB eluted as a single peak corresponding to a molecular mass of 259 kDa. Arrows show elution peaks for standards of known molecular masses (Amersham Biosciences): 1, thyroglobulin (669 kDa); 2, ferritin (440 kDa); 3, catalase (232 kDa); 4, aldolase (158 kDa); 5, BSA (67 kDa). (B) Coomassie-stained SDS-PAGE gel demonstrating that the quantities of DotB in elution fractions (Fxns) 7 to 15 correlate with the single protein elution peak. The elution profile is representative of three experiments and is identical to that for DotB K162Q (data not shown).