TABLE 1.
Plasmids
| Plasmid | Relevant features and/or genotype | Source or reference |
|---|---|---|
| pEE1 | pSU18, dcuR | This work |
| pET21a | T7 overexpression vector | Novagen |
| pMalC-2 | malE fusion overexpression vector | New England BioLabs |
| pMA1 | pRS1274 plus dcuB promoter segment (−821 to +220) | This work |
| pMA2 | pRS1274 plus dcuB promoter segment (−721 to +220) | This work |
| pMA3 | pRS1274 plus dcuB promoter segment (−616 to +220) | This work |
| pMA4 | pRS1274 plus dcuB promoter segment (−519 to +220) | This work |
| pMA5 | pRS1274 plus dcuB promoter segment (−419 to +220) | This work |
| pMA6 | pRS1274 plus dcuB promoter segment (−302 to +220) | This work |
| pMA7 | pRS1274 plus dcuB promoter segment (−219 to +220) | This work |
| pMAK8 | pRS1274 plus dcuB promoter segment (−129 to +220) | This work |
| pMA9 | pRS528 plus dcuB promoter segment (−821 to −37) | This work |
| pMA10 | pRS528 plus dcuB promoter segment (−821 to −71) | This work |
| pPG2 | dcuS dcuR | 10 |
| pPG25 | pET-21a, dcuRHis | This work |
| pPG26 | pMalC-2, malE pas dcuS202-325 | This work |
| pPG27 | pMalC-2, malE paskin dcuS202-543 | This work |
| pPG28 | pUC118 plus 709-bp dcuR-dcuB fragment | This work |
| pPG28 | pUC118 plus 709-bp dcuR-dcuB fragment | This work |
| pPG30 | pUC118 plus 365-bp dcuB fragment | This work |
| pPG31 | pMalC-2, malE kin dcuS331-543 | This work |
| pPG32 | pUC118 plus 215-bp dcuR-dcuB fragment | This work |
| pPG32 | pUC118 plus 215-bp dcuB fragment | This work |
| pPG33 | pUC118 plus 376-bp yjeA-frdA fragment | This work |
| pPG34 | pUC118 plus 324-bp f651-dctA fragment | This work |
| pPG36 | pUC118 plus 618-bp rpsV-maeA fragment | This work |
| pPG37 | pSU18, ′dcuR124-234 | This work |
| pPG38 | pUC118 plus 631-bp balA-maeB fragment | This work |
| pPG39 | pET21a plus ′dcuR124-234-His | This work |
| pRS1274 | lacZYA transcriptional fusion vector | 31 |
| pRS528 | lacZYA transcriptional fusion vector | 31 |
| pSU18 | Cloning vector | 2 |
| pT2 | pUC118 plus 402-bp yjdI-dcuS fragment | This work |
| pUC118 | Cloning vector | 36 |