TABLE 2.

List of proteins modified during heme-dependent respiration versus fermentation growth^a

Group	Spot no.	Name	Protein	Effect	Method(s)
1: Carbon metabolism	1a, 1b	GapdhB	Glyceraldehyde-3-phosphate dehydrogenase; a and b isoforms	Post-trans.	PMF, N
	2a, 2b	Pmg	Phosphoglycerate mutase; a and b proteins	Post-trans.	PMF, N
	3	PdhE1β	Pyruvate dehydrogenase E1β subunit	++	PMF, N
	4	PdhD	Pyruvate dehydrogenase D subunit	++	PMF, N
	5	LlpL	Lipoate-protein ligase	++	PMF
	6	Als	α-Acetolactate synthase	++	PMF
	7	BglA	Phospho-β-glucosidase	−−	PMF
	8	RmlB	dTDP-glucose 4,6-dehydratase	−−	PMF
	9	PurH	Bifunctional purine biosynthesis protein	++	PMF
	10	GlyA	Serine hydroxymethyltransferase	++	PMF
	11	Fhs	Formyltetrahydrofolate synthetase	++	PMF, N
2: Nitrogen metabolism	12	PepO1	Neutral endopeptidase	++	PMF
	13	PepC	Aminopeptidase C	++	PMF
	14	PepA	Glutamyl-aminopeptidase	−	PMF, N
	15	GatA	Glu-tRNA amidotransferase subunit A	++	PMF
	16	ThrC	Threonine synthase	++	PMF, N
	17	MetC	Cystathionine β-lyase	++	PMF
3: Unclassified	18	TrxB1	Thioredoxin reductase	+	PMF
	19	YgfC	Potential transcriptional regulator	++++	PMF, N
	20	YchH	Potential acetyltransferase	++	PMF
	21	TypA	GTP-binding protein	−−	PMF

^aPost-trans., posttranslational events; PMF, peptide mass fingerprinting; N, N-terminal sequencing. Greater and lesser expression under respiration compared to aeration fermentation conditions is evaluated as + to ++++ and − to −−, respectively. The number of symbols reflects the intensity of the difference observed.