Figure 3. Cellular localization of ribosome biogenesis co-factors.

A, Arabidopsis mesophyll protoplasts were co-transformed with C-terminal GFP fusion constructs indicated (left) and atFib2-mCherry (nucleolar marker). Cherry- (red), GFP- (green), chlorophyll auto-fluorescence (grey, in overlay) and DIC image is shown. Scale bar = 10 µm. B, Arabidopsis mesophyll protoplasts transformed with C-terminal GFP fusion constructs were lysed, subjected to SDS-PAGE and immunodecorated with GFP. C, Arabidopsis root tip cells were incubated with primary antibodies (left) and secondary antibody labeled with Cy2 fluorophore (green). Tissues were stained with DAPI (blue) to visualize the nucleus. Scale bar: 10 µm. D, Arabidopsis cell culture extract subjected to SDS-PAGE followed by Western Blot analysis using the indicated antibodies. White arrows point to expected migration of the protein.