FIG. 1.

Schematic diagram of the putative subdomain organization of ResE and of mutant ResE constructs. The pDG148-derived plasmids carrying resE are listed. All resE expression systems utilize the IPTG-inducible Pspac promoter and the resE SD sequence (E) or the vector-derived SD sequence (V). Numbers in parentheses represent start and end points of amino acid deletions. Solid lines show regions cloned into plasmid pDG148, and dotted lines show deleted regions. The sites and identities of amino acid substitutions in the HAMP subdomain are indicated (X). The top panel shows the conserved histidine-374 (H) and ATPase subdomains in the kinase domain and the two transmembrane subdomains (TM1 and TM2), HAMP, and PAS subdomains in the signal input domain.