FIG. 3.
Expression of nasD-lacZ and hmp-lacZ in cells grown under aerobic and anaerobic conditions. resE strains carrying the plasmid pMMN525 (full-length resE with resE SD sequence) (A and D), pMMN563 (full-length resE with vector SD sequence) (B and E), and pMMN565 (cytoplasmic resE with vector SD sequence) (C and F) were grown in 2× YT supplemented with 1% glucose and 0.2% potassium nitrate and in the absence or presence of IPTG. β-Galactosidase activities (β-gal. act.) of nasD-lacZ (A to C) and hmp-lacZ (D to F) were measured at time intervals. ○, aerobic growth in the absence of IPTG; •, aerobic growth in the presence of 1 mM IPTG; ▵, anaerobic growth in the absence of IPTG: ▴, anaerobic growth in the presence of 1 mM IPTG. Time zero indicates the end of the exponential growth.