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. 2004 Mar;186(6):1694–1704. doi: 10.1128/JB.186.6.1694-1704.2004

FIG. 5.

FIG. 5.

Induction of hmp-lacZ with NO. narGH resE strains carrying the plasmid pMMN525 (full-length resE with resE SD) (column 1), pMMN563 (full-length resE with vector SD sequence) (columns 2 and 3), pMMN534 (TM2-carrying resE with vector SD sequence) (column 4), pMMN565 (cytoplasmic resE with vector SD sequence) (column 5), pMMN564 (cytoplasmic resE lacking PAS with vector SD sequence) (column 6), and without plasmid (column 7) were grown anaerobically in 2× YT supplemented with 0.5% glucose, 0.5% pyruvate, and 1 mM IPTG (0.02 mM IPTG for the culture shown in column 2). At the mid-log phase of growth, cells were incubated for 30 min without (open columns) or with (filled columns) 10 μM NO. β-Galactosidase activity (β-gal. act.) is shown in Miller units. The data are the averages of three to five experiments with standard deviations.