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. 2013 Jan 30;8(1):e53324. doi: 10.1371/journal.pone.0053324

Figure 3. Effect of detachment procedures on B16F10-luc tumor cells.

Figure 3

OCR values (%O2/minute) (A, D) of adherent B16F10-luc and detached B16F10-luc. Trypsinized (n = 3) or collagenase group (n = 4) show a decreased oxygen consumption rate compared to control groups (n = 3 for A, n = 6 for D). Results are statistically significant (**p<0.01 for trypsinized cells, *p<0.05 for B16F10-luc+collagenase). Pictures of each treatment are displayed next to the graphs. Trypsin treatment (C) or dissolving the coating of Cytodex 3 microbeads (F) result both in cell detachment while control cells (B and E) remain adherent. Data are expressed as means ± SEM.