FIG. 2.

Activation of NerA apoenzyme by titration with FMN. Aliquots from peak 2 were supplemented with the indicated concentrations of FMN and assayed for activity. The solid line is the theoretical curve based on a simple association of the apoenzyme with FMN, with an apparent dissociation constant of 0.1 ± 0.1 μM and maximum specific activity of the fully flavinated holoenzyme of 2.6 ± 0.1 U/mg. The dashed line represents conditions in which no dissociation occurs, giving a maximum specific activity of 2.6 ± 0.1 U/mg.