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Conversion of NerA apoenzyme to holoenzyme by titration with FMN. Samples of NerA apoenzyme were treated with increasing concentrations of FMN and subjected to nondenaturing gel electrophoresis. Lanes 1 to 5 correspond to the same five FMN-treated samples that were assayed in Fig. 2. Lanes 6 and 7 are untreated samples of peaks 1 and 2 from the hydrophobic-interaction chromatography that contained fully active, yellow protein and inactive, colorless protein, respectively.
