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Reverse-phase HPLC of E. coli thiols. Traces shown are from extracts of E. coli/pJIM27 and E. coli/pJIM31 (control) cultures grown in TSB to an OD600 of 1.7 and 2.0, respectively. Arrow points to the glutathione peak. CYS, cysteine; GSH, glutathione; NAC, N-acetylcysteine; DTT, dithiothreitol; R, peak produced by chemical reagents; NEM, N-ethylmaleimide; mBBr, monobromobimane.
