FIG. 5.
Reverse-phase HPLC of S. aureus thiols. Extracts of S. aureus SH1000 and J96 (cysM) cultures grown in TSB medium to an OD600 of 1.5 were reacted with monobromobimane (mBBr) and separated by reverse-phase HPLC. These extracts are representative of all stages of growth. Control samples were reacted with N-ethylmaleimide (NEM) and monobromobimane. To identify peaks, bimane derivatives of standard thiols were used. CYS, cysteine; GSH, glutathione; NAC, N-acetylcysteine; CoASH, reduced coenzyme A; U, unidentified components; R, peaks produced by reagents. The arrow points to the cysteine peak found in SH1000 but absent in J96 (cysM) samples.