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H2 evolution catalyzed by a Synechocystis cell extract with NADH or NADPH as the electron donor. The extract (200 μg of Chl) was gassed with argon, the chamber was closed, and O2 (monitored at mass peak 32) was removed by the addition of catalase, glucose, and glucose oxidase. NADH (1 mM) (dashed line) or NADPH (1 mM) (solid line) was added at 2 min (arrow).
