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. 2013 Jan 30;8(1):e53113. doi: 10.1371/journal.pone.0053113

Figure 1. Increased membrane expression of GFP-tagged-recombinant human aquaporin 5.

Figure 1

(rhAQP5) following carbachol stimulation in HSG cells. (A) M3R expression was detected in HSG cells (red color) using polyclonal (a, b) or monoclonal (c) antibody by immunocytochemistry. Stained cells were observed at ×200 (a) and ×400 (b, c) magnifications. (B) hAQP5 protein expressions were examined by western blotting in total HSG cell lysates purified from non- or rhAQP5 vector-transfected HSG cells (a) and in HSG cell membrane fractions purified from transfected cells with or without CCh treatment (b) in duplicates #1 and #2. (C) To confirm the hAQP5 trafficking in HSG cells, control GFP (a) or rhAQP5 (c) vector transfected HSG cells were treated with carbachol (CCh, 100 μM) (a and c) or no stimulation (b). Images were taken under microscope with a ×400 magnification using a Zeiss Axiovert 200M microscope and obtained with AxioVs40 software (Ver. 4.7.1.0, Zeiss).