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. 2013 Jan 30;8(1):e53113. doi: 10.1371/journal.pone.0053113

Figure 6. Inhibition of CCh-induced hAQP5-GFP transfected HSG cell shrinkage by SjS plasma.

Figure 6

(A) hAQP5-GFP transfected HSG cells were pre-incubated with HC or SjS plasma for 24 hours and stimulated with CCh. Representative still images were captured before and after 30 minutes of CCh treatment. (B) Cell volume changes were quantified by measuring the areas within the cell membrane before and after CCh stimulation and normalized by the area before stimulation. Cross section of the cell at center of z-axis was considered for the analysis. Data are based on three independent experiments and all cells under a microscopic view were counted for calculation each time. Each column represents the mean ± SEM, p value less than 0.05 was considered significantly different by two-tailed unpaired Student's t-test, *p<0.05.