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The growth phenotype of the PE-deficient mutant CS111 is partly rescued by choline supplementation to the medium. S. meliloti 1021 (solid lines, closed symbols) and the Pss-deficient mutant CS111 (dashed lines, open symbols) were grown in MOPS minimal medium without choline supplementation (circles) or in the presence of 10 μM choline (diamonds), 100 μM choline (squares), or 1 mM choline (triangles).
