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Stimulation of in vitro phospholipid N-methyltransferase activity by added PEa
| Strain | Incorporation of methyl groups (pmol/mg of protein/min)
|
||
|---|---|---|---|
| Without PE | With PE | Stimulation (x-fold) | |
| Wild type | 0.71 | 23.91 | 33.7 |
| KDR309 | 0.13 | 0.78 | 6.0 |
| KDR310 | 0.23 | 6.24 | 27.1 |
The assay for phospholipid N-methyltransferase was done with cell-free extracts of S. meliloti wild-type 1021, Pmt-deficient mutant KDR309, and PE-deficient mutant KDR310 after growth in complex medium.
