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. 2004 Mar;186(6):1629–1637. doi: 10.1128/JB.186.6.1629-1637.2004

FIG. 6.

FIG. 6.

tcuRABC functions are all required for tricarballylate catabolism. In all cases, pBAD30 refers to the empty cloning vector, and recA refers to allele recA1. Cells were grown aerobically in NCE medium containing tricarballylate (20 mM) with the exception of those for panel C, where 1 mM glycerol was also added to the medium. (A) Complementation of a tcuR mutant. Open diamonds, growth response of a tcuR+ strain (JE4271); open triangles, growth response of a tcuR strain (JE6329); solid circles, growth response of a tcuR strain (JE6329) with tcuR+ provided in trans (pTCU18) in medium lacking l-(+)-arabinose; solid triangles, growth response of the same strain in medium containing 500 μM l-(+)-arabinose. (B) Complementation of a tcuA strain. Open diamonds, growth response of a tcuA+ recA strain (JE2397); open triangles, growth response of a tcuA recA strain (JE7586); solid circles, growth response of a tcuA recA strain (JE7586) with tcuA+ provided in trans (plasmid pTCU8) in medium lacking l-(+)-arabinose; solid triangles, growth response of the same strain in medium containing 500 μM l-(+)-arabinose. (C) Marker rescue of a tcuB strain. Open diamonds, growth response of a tcuB+ strain (JE4271); open triangles, growth response of a tcuB strain (JE6335); solid triangles, growth response of a tcuB strain (JE6335) with tcuB+ provided in trans (plasmid pTCU19). (D) Complementation of a tcuC strain. Open diamonds, growth response of a tcuC+ recA strain (JE2397); open triangles, growth response of a tcuC recA strain (JE7585); solid circles, growth response of a tcuC recA strain (JE7585) with tcuC+ provided in trans (plasmid pTCU5) in medium lacking l-(+)-arabinose; solid triangles, growth response of the same strain in medium containing 500 μM l-(+)-arabinose.