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. 2013 Jan 30;8(1):e54927. doi: 10.1371/journal.pone.0054927

Figure 3. Mapping the 3rd Ventricle Stem Cell Niche, weanling vs. adult.

Figure 3

Panel A displays the nestin-delineated subventricular zone (SVZ), which served as an internal positive control. Images in series B were acquired from a PND21 male rat and images in series C were taken from an adult male (PND110) rat, demonstrating the nestin-positive region/cells (red label) that extended into the parenchyma of the hypothalamus (∼0.1 mm or more from the ventricular wall) at the rostral end of the 3rd ventricle, namely the 3rd ventricle stem cell niche (3VSCN). Distance between the 2 adjacent brain slices is 90 µm. SVZ, subventricular zone; OC, optic chiazma 3V, the 3rd ventricle; blue labelings, DAPI-staining.