Figure 5. Representative images of the triple labeling of calbindin D28k (CB28), Ki67, and DAPI.

Four sets of sequential images are displayed defining the SDN-POA (CB28-delineated, green fluorescence) and proliferative cells (Ki67-labeled, red fluorescence) in the hypothalamic preoptic area. The blue (DAPI) fluorescence delineates cell nuclei. The four series of images are from a PND 21 male (A1–D1), PND 21 female (A2–D2), PND 110 male (A3–D3), and PND 110 female (A4–D4). A to C images are aligned in a direction from rostral to caudal with 90 µm between adjacent slices along the longitudinal axis. Images displayed under higher magnification in D were acquired at the locations indicated by the white dotted area in their corresponding series.