FIG. 2.
Effect of different mutations on frameshifting. The slippery sequence is shown in bold, its point mutations are underlined, and the deletion is included in a box. In these experiments, orf5 (and its variants) was placed under the control of the P10 promoter of T7 and overexpressed in vivo in the presence of a 35S-labeled amino acid mix and rifampin. The labeled products were separated by SDS-12% PAGE and autoradiographed. The positions of the bands correspond to the sizes of gp5A and gp5B prior to processing of their 123 amino-terminal residues.