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. 2013 Jan 30;8(1):e54701. doi: 10.1371/journal.pone.0054701

Figure 4. The effect of silencing and overexpressing the high affinity glucose transporter (Hxt) gene on the ability of Fusarium oxysporum strain 11C to colonise and release ethanol from a straw/bran mix (10∶1 ratio).

Figure 4

(A) Gene silenced mutants pSilent-1-Hxt-1, 3, 5 & 7, mutant pSilent-1-A transformed with the empty silencing vector and wild type fungus; (B) gene overexpressed mutants pBARGPE1-Hxt-1, 5, 6 & 9, mutant pBARGPE1-1 transformed with the empty overexpression vector and wild type fungus. For both A and B, fungi were cultured on straw/bran for 96 h aerobic followed by 96 h oxygen-limiting growth. Ethanol was estimated using QuantiChrom™ Ethanol Assay Kit (DIET-500) (BioAssay Systems, USA). Results are based on two experiments, each with three replicates per strain/mutant. Bars indicate SEM [LSD0.05 A = 11.9 (ethanol), non-significant (biomass); LSD0.05 B = 15.16 (ethanol), non-significant (biomass)].