Figure 7. Kinetics of glucose uptake by wild type Fusarium oxysporum strain 11C, overexpression mutant pBARGPE1-Hxt-6 and PTGS mutant pSilent1-Hxt-3.

(A) Uptake of D-glucose and D-xylose (nM of glucose/xylose transported per 10⁶ cells) by F. oxysporum. The amount of sugar used in each transport assay is indicated above each graph. Uptake was measured as described by Jørgensen et al. [58]. Results are based on two experiments, each with three replicates per strain/mutant per medium. Bars indicate the SEM. (B) Double-reciprocal plots were plotted to deduce the Km_(glucose) and Vmax values for the F. oxysporum hexose transporter (Hxt). The inset is an amplification of the 1/S values from 0 to 0.001 µM. S, Substrate concentration (µM); V, velocity (nM/10⁶ cells per min). Bars indicate SEM.