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. 2013 Jan 30;8(1):e54788. doi: 10.1371/journal.pone.0054788

Figure 3. Schedule for the administration of the TNF-αinhibitor etanercept and the effects of etanercept on the 5-FU-induced upregulation of inflammatory cytokine gene expression in the colons of mice.

Figure 3

A) Thirty minutes before the administration of 5-FU, etanercept (5 mg/kg, s.c.) or vehicle (saline, s.c.) was administered on Days 0 and 3. Twenty-four hours after the final administration of 5-FU, experiments were performed. The upregulated gene expressions of TNF-α (B and G), IL-1β (C and H), IL-6 (D and I), IFNγ (J), IL-17A (E and K) and IL-22 (F and L) by 5-FU were significantly inhibited by the administration of etanercept in the transverse (B-F) and distal (G-L) colons. Each column represents the mean ± S.E.M. of 4–6 independent experiments. *p<0.05, **p<0.01 and ***p<0.001 vs. Saline+saline. #p<0.05, ##p<0.01 and ###p<0.001 vs. Saline +5-FU.