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Time lapse expression of the sar P1-gfp reporter in a flow cell biofilm of S. aureus MN8(pJY209). The images were acquired at 15-min intervals by using identical CSLM settings with a 20× objective lens. Green indicates those cells expressing the reporter, and the remainder of the biofilm appears red from treatment with propidium iodide. Sequential images taken every 90 min are shown, with the number of hours postinoculation indicated.
