TABLE 2.

Intrinsic rate constants, r_a, and goodness of fit expressed as χ²/DF for the interaction of SCWP with immobilized 3SLH calculated on the basis of the model for bivalent binding to fixed ligands (27)

Flow rate (μl/min)	ka (M−1 · s−1)	kd (s−1)	Kd (M)a	ra (nm)	χ2/DF
10	3.13 × 104	2.50 × 10−2	7.97 × 10−7	13.2	20.7
50	5.24 × 104	3.89 × 10−2	7.42 × 10−7	14.4	31.2

^aEquilibrium dissociation constant calculated as k_d/k_a.