TABLE 3.

Intrinsic rate constants and goodness of fit expressed as χ²/DF from fitting of the heterogeneous-ligand model for three parallel reactions to the data measured at the indicated flow rate for binding of 3SLH to SCWP immobilized on a sensor chip at a low ligand density

Binding site	10 μl/minb			50 μl/minc
	ka (M−1 · s−1)	kd (s−1)	Kd (M)a	ka (M−1 · s−1)	kd (s−1)	Kd (M)a
1	1.77 × 106	9.08 × 10−5	5.13 × 10−11	2.89 × 106	2.36 × 10−4	8.17 × 10−11
2	4.20 × 104	2.69 × 10−3	6.40 × 10−8	6.42 × 104	3.70 × 10−3	5.76 × 10−8
3	4.22 × 104	1.17	2.77 × 10−5	1.73 × 104	4.09 × 10−1	2.36 × 10−5

^aEquilibrium dissociation constant calculated as k_a/k_d.

^bχ²/DF, 0.81.

^cχ²/DF, 0.45.