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Primer extension analysis of the divergently transcribed ulaG and ulaA genes. The primed-extended products using total RNA of strain JA211 growing aerobically on CAA (lanes 1) were electrophoresed with a sequencing ladder (lanes A, C, G, and T) generated by using the same template and primer. A portion of the nucleotide sequence deduced from the sequencing lanes is shown. The extended product assigned as the 5′ end of each gene is labeled with an asterisk.
