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Binding of UlaR to ulaG and ulaA promoters. The line over the panels indicates the fragment corresponding to the full-length ulaG and ulaA promoter region used as a probe. The 32P-labeled DNA fragment was added at 2.5 (A) or 0.25 (B) nM to binding mixtures containing different amounts of purified UlaR protein. The migration patterns of the different DNA-protein complexes are indicated.
