FIG. 6.

Interactions of CRP and IHF with different fragments of the ulaG-ulaA intergenic region. (A) Location of the putative IHF and CRP binding sites in the intergenic region. The sequence of the CRP site is indicated, and above it the arrowheads indicate the nucleotides changed by site-directed mutagenesis in the CRP site. (B) Binding of cAMP-CRP to probe P65, using 2 (lanes 2 and 3) or 10 (lanes 4 and 5) μg of crude extracts of strain JA211 transformed with pBS-CRP, in the absence (lanes 2 and 4) or presence (lanes 3 and 5) of 125 μM cAMP. No extract was added to lane 1. (C) Binding of IHF to probe P100 using extracts of strain JA134 (lane 1) and JA184 (lane 2) and no extract (lane 3).