TABLE 1.
Bacterial strains and plasmids used in this work
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| XL1Blue | recA1 lac endA1 gyrA96 thi hsdR17 supE44 relA1 (F′ proAB lacIq lacZΔM15 Tn10) | Stratagene |
| TE2680 | F− λ− IN(rrnD-rrnE) ΔlacX74 rplS galK2 recD::Tn10d-tet trpDC700::putA13033::(Kanr Cmrlac) | 10 |
| JMH0317 | W3110 rpoS::Tn10 | J. Membrillo-Hernandez |
| JA134 | ECL1 Lyx+ | 25 |
| JA182 | JA134 Δ(tyrR-fnr-nac-trg)17 zdd-230::Tn9 | 14 |
| JA184 | JA134 himA::cat | 14 |
| JA185 | JA134 himD3::cat | 14 |
| JA188 | JA134 rpoS::Tn10 | This work |
| BL21(DE3) | E. coli B F−ompT hsdS (rB− mB−) gal dcm λ(DE3) | Pharmacia Biotech |
| JA211 | JA134 yiaR::catΔulaR | 6 |
| JA216 | JA211 crp::cat | This work |
| Plasmids | ||
| pRS550/pRS551 | Apr Kmr; contains promoterless lacZYA | 28 |
| pET43c (+) | Nus tag bla (Carbr) lacI | Novagen |
| pET-UlaR | ulaR in pET43c (+) | This work |
| pBS-CRP | crp in pBluescript | This work |