Figure 3. Effects of RhoC overexpression on promoting proliferation and colony formation of HL7702 cells.

Silver nitrate staining analysis of cell proliferation capability (magnification: × 400; A) and quantitative data (B). For the colony formation assay, at least three microscopic fields were randomly selected and the number of darkly stained silver granules per cell was counted. The average number was presented. The colony formation rate in culture dishes (C) or in soft agar (D) was calculated from three independent experiments. *P<0.05 compared with EV control.