Table 2. Identification of belinostat metabolites in human plasma using HPLC-DAD & LC-MS/MS analyses.
Peak ID | Retention Time (min) | λ (nm) | [M+H] + | MS/MS | Proposed Structure |
Belinostat | 11.5 | 268 | 319 | 93, 204, 268, 286 | Parent compound |
M1 | 16.8 | 268 | 495 | 93, 204, 268, 286, 319 | Belinostat glucuronide |
M2 | 12.1 | 268 | 303 | 93, 204, 268, 286 | Belinostat amide |
M3 | 13.0 | 268 | 333 | 93, 204, 268, 286 | Methyl belinostat |
M4 | 15.1 | 268 | 304 | 93, 268, 286,319 | Belinostat acid |
M5 | 10.9 | 268 | 481 | 93, 204, 268, 286, 319 | Belinostat glucoside |