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LPS phenotype from wild-type and wbbO mutant strains of K. pneumoniae O1. Periodic acid-silver stain (left panel) of LPS separated by SDS-PAGE. CWK2 is an O1:K− serotype strain used as a positive control. Compared to wild type, the wbbO mutant strain has lost its O antigen and only rough LPS remains. Immunoblots (center and right panels) of the same material developed with antibody that is specific for either the d-galactan I or d-galactan II components.
