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. 2004 Mar;72(3):1265–1274. doi: 10.1128/IAI.72.3.1265-1274.2004

FIG. 3.

FIG. 3.

Coomassie blue-stained gel (top) and Western blot (bottom) of crude membrane preparations. (A) Lanes 1 to 4, M. smegmatis mc2155 transformants containing pMV361, pRDIII320, pRDIII320Δ2, and pRDIII320Δ1, respectively. (B) M. smegmatis (lane 1) and M. avium subsp. paratuberculosis (lane 2). The arrows indicate the position of the MptC protein detected by the serum raised against the recombinant fusion protein. The numbers on the left indicate the positions of protein markers in kilodaltons.