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Peptide aMptD-mediated capture PCR from M. avium subsp. paratuberculosis-negative (panels 1 and 2) and -positive (panels 3 and 4) bulk milk samples with ISMav2-derived primers. Lanes a and b, products of PCRs of the sample; lanes c, internal positive control (sample spiked with M. avium subsp. paratuberculosis DNA). The arrow indicates the expected position of the PCR product in base pairs.
