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. 2004 Mar;72(3):1223–1229. doi: 10.1128/IAI.72.3.1223-1229.2004

FIG. 3.

FIG. 3.

LPS-stimulated newborn monocytes have lower amounts of TLR-4 and CD14 than adult cells. (A) Leukocyte-rich plasma from adults and newborns was left untreated (Control) or stimulated with 5 ng of LPS/ml for 30 min at 37°C and then stained with a mouse anti-TLR-4 MAb and then an FITC-conjugated goat anti-mouse IgG before being assessed by FACS. The mean ± the SD fluorescent intensity for adult and newborn monocytes is shown. (B) Adult venous blood or full term newborn cord blood were incubated with 0, 0.1, or 10 ng of LPS/ml for 30 min at 37°C, after which the samples were stained with anti-CD14 MAb (MY4) or an isotype control antibody. The cells were then incubated with FITC-conjugated rabbit anti-mouse IgG for 30 min at 4°C and then assayed by FACS. The results are expressed as mean ± the SD fluorescent intensity for adult (•) and newborn (○) monocytes. ✽, P < 0.05; ✽✽, P < 0.01 (adult versus newborn).